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The effects of FC on SMC and macrophage-related gene expression. Subconfluent mouse SMCs were pretreated overnight with or without an ACAT inhibitor F-1394 (1 μM), then treated with (Chol, Chol + ACAT inhibitor) or without (Control, ACAT inhibitor alone) Chol:MβCD (10 μg/ml) in 0.2% BSA (72 h) in the presence of F-1394. Total RNA was extracted and subjected to QRT-PCR analysis. All data are averages ± SEM from independent duplicates. *,α-actin was not detected. †, P < 0.01 vs. Chol.
