Table 1. Primer and probe sequences for QRT-PCR.
Genes | Forward primer | Reverse primer | Probe |
---|---|---|---|
HMG-CoA reductase | GGGAGCATAGGCGGCT | TGCGATGTAGATAGCAGTGACA | CAACGCCCACGCAGCAACA |
Calponin H1 | AGTACTGCCTGAACCCGGAGT | GTTGTGCGGGTGGTGATTG | CCCAGAGCTGAGTGAGCCCACCC |
Myosin heavy chain | CATGGACCCGCTAAATGACA | CAATGCGGTCCACATCCTTC | TCACTCCTCAATGCCTCCTCTGACAAGTTT |
α-Tropomyosin | AGCTCGACAAAGAGAACGCC | ATCTTCCAGCTGCTTGCTCC | TGGATCGAGCTGAGCAAGCGGA |
Mac-2 | AGGAGAGGGAATGATGTTGCC | GGTTTGCCACTCTCAAAGGG | TCCACTTTAACCCCCGCTTCAATGAGA |
All sequences are from 5′ to 3′. The probes are labeled at the 5′ and 3′ positions with 6-carboxyfluorescein reporter and 6-carboxytetramethylrhodamine quencher, respectively.