. Author manuscript; available in PMC: 2009 Dec 1.

Published in final edited form as: Hum Immunol. 2008 Oct 26;69(12):815–825. doi: 10.1016/j.humimm.2008.09.009

Table 1. CD8⁺ T cell epitopes analyzed in this study and their conservation in Dryvax®.

peptide name	gene name and (a.a. position)^a	a.a. sequence	restricting allele	conservation in Dryvax®	temporal expression^b	reference
A1H1	A24R (278–286)	ITDFNIDTY	A*0101	Yes	E	[16]
A1H2	B8R (139–147)	DMCDIYLLY	A1 supertype	Yes	E	[14]
A1H3	B8R (153–162)	FGDSKEPVPY	A1 supertype	Yes	E	[14]
A1H4	B8R (262–271)	FLSMLNLTKY	A1 supertype	Yes	E	[14]
A1H5	C10L (297–305)	SQSDTVFDY	A1 supertype	Yes	E	[14]
A1H6	C10L (298–306)	QSDTVFDYY	A1 supertype	Yes	E	[14]
A1H7	C12L (326–334)	VYINHPFMY	A*2902	Yes	E	[16]
A1H8	D1R (156–164)	FTIDFKLKY	A1 supertype	Yes	E	[14]
A1H9	D12L (11–19)	GTHVLLPFY	A1 supertype	Yes	E	[14]
A1H10	F11L (259–267)	CMLTEFLHY	A1 supertype	Yes	E	[14]
A1H11	VACWR008 (29–38)	VSVNNVCHMY	A1 supertype	Yes	E	[14]
A1H12	VACWR008 (104–112)	QSITRSLIY	A1 supertype	Yes	E	[14]
A1H13	VACWR013 (97–106)	VTDTNKFDNY	A1 supertype	VTDTNKFAHY	E	[14]
A1H14	A27L (89-103)^c	LRAAMISLAKKIDVQ	A1	Yes	L	[18]
A2H1	A6L (172–180)	ILSDENYLL	A2 supertype	Yes	L	[14]
A2H2	A7L (342–350)	FLVIAINAM	A2 supertype	Yes	L	[14]
A2H3	A36R (1–9)	MMLVPLITV	A2 supertype	Yes	E	[14]
A2H4	A47L (169-177)	LLYAHINAL	A*0201	Yes	IE	[12]
A2H5	A55R (78–86)	YIYGIPLSL	A2 supertype	Yes	E	[14]
A2H6	B22R/C16L (60-68)	CLTEYILWV	A*0201	Yes	?^d	[12]
A2H7	C7L (74–82)	KVDDTFYYV	A*0201	Yes	E	[12]
A2H8	E2L (249–257)	KIDYYIPYV	A2 supertype	Yes	E	[14]
A2H9	E9L (107–115)	FLNISWFYI	A2 supertype	Yes	E	[14]
A2H10	F12L (286–295)	NLFDIPLLTV	A2 supertype	Yes	E	[14]
A2H11	F12L (404–412)	FLTSVINRV	A2 supertype	Yes	E	[14]
A2H12	G5R (18–26)	ILDDNLYKV	A2 supertype	Yes	IE	[14]
A2H13	H3L (184-192)	SLSAYIIRV	A*0201	Yes	L	[11]
A2H14	I4L (720–728)	SMHFYGWSL	A2 supertype	Yes	E	[14]
A2H15	N2L (93–101)	YVNAILYQI	A2 supertype	Yes	IE	[14]
A2H16	O1L (247–255)	GLNDYLHSV	A2 supertype	Yes	IE	[14]
A2H17	VACWR148 (177–186)	YLYTEYFLFI	A2 supertype	YLYTEYFLFL^e	L	[14]
A2H18	A55R (391-399)	AMLNGLIYV	A*0201	Yes	E	[55]
A2H19	B5R (5-19)^c	SVVTLLCVLPAVVYS	A2	Yes	E/L	[18]
A2H19′	B5R (8-16)^f	TLLCVLPAV
A3H1	A8R (79–88)	AVKDVTITKK	A3 supertype	Yes	IE	[14]
A3H2	A31R (86–94)	VTSSGAIYK	A3 supertype	Yes	E	[14]
A3H3	B6R (154–163)	GTIAGGVCYY	A3 supertype	Yes	E	[14]
A3H4	C5L (158–166)	KVMFVIRFK	A3 supertype	Yes	E/L	[14]
A3H5	C7L (31–40)	KLKIISNDYK	A3 supertype	Yes	E	[14]
A3H6	C9L (193–201)	ATSLDVINY	A3 supertype	Yes	E/L	[14]
A3H7	D1R (152–161)	KTKNFTIDFK	A3 supertype	Yes	E	[14]
A3H8	D5R (670–678)	YLLVKWYRK	A3 supertype	Yes	E	[14]
A3H9	G8R (65–73)	IVFNLPVSK	A3 supertype	Yes	?^g	[14]
A3H10	I3L (116–124)	AVYGNIKHK	A3 supertype	Yes	IE	[14]
A3H11	J6R (332–340)	NQVKFYFNK	A3 supertype	Yes	E	[14]
A3H12	VACWR013 (93–102)	KVLHVTDTNK	A3 supertype	Yes	E	[14]
A3H13	VACWR195 (74–83)	AVFKDSFLRK	A3 supertype	Yes	IE	[14]
A24H1	A48R (58–66)	TYNDHIVNL	A*2301	Yes	IE	[16]
A24H2	C6L (54–63)	RYYDGNIYE	A24 supertype	RYYDGNIYD	IE	[14]
A24H4	D5R (349–357)	VWINNSWKF	A24 supertype	Yes	E	[14]
A24H5	D5R (663–672)	RYRFAFLYLL	A24 supertype	Yes	E	[14]
B7H1	B22R/C16L (53-61)	TVADVRHCL	B7	Yes	?^d	[17]
B7H2	C1L (102–111)	KPKPAVRFAI	B7 supertype	Yes	E	[14]
B7H3	D1R (686–694)	HPRHYATVM	B7 supertype	Yes	E	[14]
B7H4	F4L (6–14)	APNPNRFVI	B7 supertype	Yes	E	[14]
B7H5	J2R (62-70)	EATKLCDVL	B*3502	Yes	E	[17]
B7H6	J6R (303-311)	MPAYIRNTL	B7 supertype	Yes	E	[14]
			B7			[17]
B7H7	O1L (335–344)	RPMSLRSTII	B7 supertype	Yes	IE	[14]
B44H1	A3L (90–98)	DEVASTHDW	B*4403	Yes	L	[16]
B44H2	A3L (264–272)	YEFRKVKSY	B*4403	Yes	L	[16]
B44H3	A23R (287–295)	HDVYGVSNF	B*4403	Yes	E	[16]
B44H4	B8R (110–118)	TEYDDHINL	B44 supertype	Yes	E	[14]
B44H5	C3L (120–128)	GESKSYCEL	B44 supertype	Yes	L	[14]
B44H6	C7L(56-64)	DEVKGLTVF	B*1801	Yes	E	[19]
B44H7	D1R (126–134)	EERHIFLDY	B*4403	Yes	E	[16]
B44H8	D5R (298–306)	LENGAIRIY	B*4403	Yes	E	[16]
B44H9	D5R (691–699)	EEIPDFAFY	B*4403	Yes	E	[16]
B44H10	E3L (86–94)	DDVSREKSM	B*4403	Yes	IE	[16]
B44H11	G2R (181–189)	DELVDPINY	B44 supertype	Yes	E	[14]
B44H12	I3L (173–181)	IEGELESLS	B*4403	Yes	IE	[16]
B44H13	M2L (38–46)	AELTIGVNY	B*4403	Yes	IE	[16]
B44H14	F14.5L (41–49)^h	EEQELLLLY	B*4403	Yesⁱ	E	[16]
B44H15	VACWR013 (21–29)	DEIKCPNLN	B*4403	VETKCSNLD^j	E	[16]
B44H16	B5R (105-119)^c	TKYFRCEEKNGNTSW	B44	Yes	E/L	[18]

: Gene names are based on nomenclature for Copenhagen strain. When not conserved in Copenhagen strain, nomenclature for WR strain is used. Misidentification of the genes in the original publications are corrected.

: Based on the reference [48].

: Minimal epitope was not determined

: B22R/C16L was not analyzed

: When compared with the WR sequence, the C-termial “I” in the original paper should be “L”

: Predicted HLA-A2 binding motif in B5R (5-19) peptide by HLA Peptide Binding Predictions (http://www.bimas.cit.nih.gov/molbio/hla_bind/) [56] and SYFPEITHI (http://www.syfpeithi.de/Scripts/MHCServer.dll/EpitopePrediction.htm)[57]

: G8R gene did not fit any class

: Also known as F3

ⁱ

: ORF was identified by BLAST search on the genomic DNA sequences

: Not conserved only in Acambis 3 strain

Table 1. CD8+ T cell epitopes analyzed in this study and their conservation in Dryvax®.

Table 1. CD8⁺ T cell epitopes analyzed in this study and their conservation in Dryvax®.