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Nonconsecutive serial sections through the cardiac outflow tract from a 9-day embryo that had been treated with HcysTD at three time points during the formation of the aorticopulmonary septum (incubation days 2–4). The septum has separated the aorta (a) from the pulmonary trunk (p) down to the level shown on the left; but a few sections upstream (toward the heart), there is an obvious subaortic VSD (arrowhead).
