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. Author manuscript; available in PMC: 2009 Feb 10.
Published in final edited form as: Mol Pharmacol. 2008 Jun 24;74(3):654–661. doi: 10.1124/mol.108.046201

Fig. 3.

Fig. 3

EF24 impairs TNF-α induced NF-κB nuclear translocation. A549 cells were grown in 96-well plates and treated with TNF-α or control (DMSO) for 30 min before sample processing for the detection of NF-κB as described under Materials and Methods (top two rows of a). The induction of NF-κB nuclear translocation by TNF-α was quantified as shown in b, with an apparent EC50 value of 1.5 ng/ml. The effect of EF24 or cucumin treatment (30 min) on TNF-α-induced nuclear translocation of NF-κB was shown in the lower two rows in a and quantified as shown in c.