Skip to main content
. Author manuscript; available in PMC: 2009 Feb 10.
Published in final edited form as: Mol Pharmacol. 2008 Jun 24;74(3):654–661. doi: 10.1124/mol.108.046201

Fig. 6.

Fig. 6

EF24 inhibits IKKβ kinase activity in a reconstituted system. Recombinant IKKβ was incubated with increasing concentrations of EF24 (a) or curcumin (b) for 15 min. Addition of Mg/[γ-32P] cocktail with purified GST-IκB started the reactions, which were continued for 15 min at 30°C. Proteins were separated by SDS-PAGE and processed for radiolabeled GST-IκB (top), total GST-IκB (middle), and IKKβ (bottom) as described in legend to Fig. 5. Controls include reactions without IKKβ (lane 1) or without IκB (lane 2).