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. 2003 Oct 29;100(23):13561–13566. doi: 10.1073/pnas.1835863100

Fig. 5.

Fig. 5.

Immunoblot analysis of EhNP and recombinant COX-like protein. Polyclonal antibodies against the COX-like protein detected the 72- and 66-kDa protein bands in EhNP and recombinant COX-like protein. Lane 1, 300 ng of active sheep COX-1 enzyme; lane 2, 300 ng of sheep COX-2 enzyme; lane 3, 100 μg of EhNP; lane 4, 100 μg of Eh cytosol protein; lane 5, 50 μg of recombinant ameba COX-like protein; lane 6, 300 ng of active sheep COX-1 enzyme; lane 7, 300 ng of sheep COX-2 enzyme.