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Inhibition of COX-like antibody binding in the presence of excess EhNP or COX-like protein. One hundred micrograms of EhNP (A) and 50 μg of recombinant COX-like proteins (B) were resolved in 10% SDS/PAGE and competed with excess proteins as indicated. Excess proteins (0×-100×) were preincubated with polyclonal antibodies against the COX-like protein for 30 min before probing the blot.
