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Expression of hTERT, SV40 T antigens, and Ras. Total cell lysates were prepared from four HDF (TIG-1, TIG-3, BJ, and IMR-90) infected with retroviral vectors expressing hTERT (T), SV40 ER (S), and H-RasV12 (R), from REF infected with retroviral vectors expressing S and R, and from uninfected TIG-3. Ten micrograms of each total cell lysate was subjected to immunoblot analysis with the antibodies indicated on the right.
