Fig. 1.

BDGF analysis of HCMV ORFs. All previously annotated ORFs of HCMV are listed from high (upper left) to low (lower right) normalized BDGF (N-BDGF) scores. Expression of the ORF was scored as positive if a report exists in the published literature directly demonstrating the expression of a protein or showing that a mutation within the ORF generates a viral growth phenotype. NR indicates that no report was found. The presence of orthologues corresponding to HCMV ORFs within the genomes of chimpanzee cytomegalovirus (CCMV), rhesus cytomegalovirus (RhCMV), and murine cytomegalovirus (MCMV) are indicated. Green boxes designate characteristics favoring the conclusion that an ORF encodes a protein, and red boxes mark characteristics arguing that an ORF does not encode a protein. The length of the polypeptide potentially encoded by each ORF and the presence of a Kozak translational initiation motif (Kozak AUG) are provided for informational purposes. The UL65 ORF was reported to be expressed (26), but the reported polypeptide does not match the UL65 sequence (3).