Figure 3.

Accumulation of Ub-proteins in fast (gastrocnemius) muscle of GAA KO and AD-GAA KO. Muscle lysates, prepared as detergent (Triton X-100)-soluble and -non-soluble fractions, were analyzed by immunoblotting with anti-ubiquitin (FK2) antibody. (A) Muscle samples were taken from ∼4-month-old WT, GAA KO and AD-GAA KO mice. High molecular mass Ub-proteins are marked by a bracket. Data shown are representative of at least four independent experiments. (B) Age-dependent accumulation of soluble and non-soluble Ub-proteins in muscle from young (6-week-old; I) and old (10-month-old; II) GAA KO. For each time point and each genotype, three mice were used.