Fig. 3.

Localization of the transcriptional start sites of trxB and yocJ. Cells of JH642 were grown in TSS medium to midlogarithm, and the cultures were split. Diamide was added to one culture. Cells were harvested after 30 min, and RNA was purified for primer extension reaction. Primer extension products by using the primer specifying the trxB transcript and the yocJ transcript, respectively, are shown. Lane 1, -diamide; lane 2, +diamide. Four lanes on the left in A and B are the gel profiles of sequencing reactions A, G, C, and T.