FIG. 6.

Effects of PI3 kinase inhibitors on GSH synthesis and Nrf2 activity in primary astrocytes. Astrocytes were treated with PI3 kinase inhibitors, LY294002 (A) and wortmannin (B), for 16 and 6 h, respectively. Total GSH was measured by HPLC. (C) The Nrf2 activity was measured by transient transfection with the ARE reporter plasmid followed by the luciferase assay. Cells were treated with LY294002 at indicated concentrations. Each point represents the average of three separate experiments performed in duplicate (mean ± SE) (**p < 0.01; significantly different from control untreated cells; one-way ANOVA and Dunnett post hoc test).