FIG. 1.

Ni and MALP-2 interact to promote release of VEGF, CXCL1 and from HLF. Cells were stimulated with 200μM Ni and 600 pg/ml MALP-2 alone or combined for 48 h as described under “Materials and Methods.” Conditioned medium was analyzed using specific enzyme-linked immunoassays for (A) VEGF, (B) CXCL1, (C) CXCL5. Data shown are mean ± SEM and are representative of three to five independent experiments performed in triplicate. ***Different from untreated control (p < 0.001); Δ, different from both Ni and MALP-2 treatment alone (p < 0.01).