TABLE 1.

Effects of Ni and MALP-2 on Angiogenesis-Related Gene Expression in HLF^a

	Ni	MALP-2	Ni + MALP-2		Ni	MALP-2	Ni + MALP-2
AKT1	0.9 ± 0.1	0.9 ± 0.1	1.0 ± 0.1	IGF1	0.5 ± 0.1	1.0 ± 0.2	0.5 ± 0.1
ANGPT1	1.1 ± 0.2	0.9 ± 0.1	1.2 ± 0.1	IL1B	2.6 ± 1.1	1.4 ± 0.4	1.9 ± 0.4
ANGPT2	1.9 ± 1.1	0.9 ± 0.2	1.2 ± 0.2	IL6	2.1 ± 0.4*	1.4 ± 0.1	7.1 ± 0.4*
ANGPTL3	2.4 ± 0.3	1.1 ± 0.3	3.1 ± 1.5	ITGAV	0.6 ± 0.1	1.0 ± 0.2	0.8 ± 0.3
ANGPTL4	25.0 ± 9.9*	0.6 ± 0.2	34.6 ± 14.7*	ITGB3	1.5 ± 0.5	1.1 ± 0.3	2.0 ± 0.6
ANPEP	0.8 ± 0.1	1.1 ± 0.1	0.9 ± 0.2	JAG1	2.0 ± 0.4	0.9 ± 0.1	1.8 ± 0.1
BAI1	1.5 ± 0.5	1.0 ± 0.4	1.7 ± 1.1	KDR	1.9 ± 0.3	1.2 ± 0.5	1.6 ± 0.3
CCL11	0.4 ± 0.1*	1.4 ± 0.2	0.6 ± 0.1	LAMA5	1.3 ± 0.3	0.9 ± 0.1	1.2 ± 0.2
CCL2	1.4 ± 0.2	2.6 ± 0.1*	3.7 ± 0.2*	LECT1	2.6 ± 0.7	0.7 ± 0.1	1.5 ± 0.3
CDH5	3.2 ± 0.6	0.8 ± 0.1	1.7 ± 0.2	LEP	8.2 ± 4.7*	0.8 ± 0.1	6.6 ± 3.5*
COL18A1	0.7 ± 0.1	0.8 ± 0.2	0.6 ± 0.1	MDK	0.8 ± 0.1	1.3 ± 0.1	1.0 ± 0.1
COL4A3	1.1 ± 0.1	1.1 ± 0.2	1.1 ± 0.2	MMP2	0.9 ± 0.3	1.0 ± 0.1	1.0 ± 0.2
CXCL1	8.7 ± 3.6*	34.8 ± 6.9*	104.3 ± 16.1*	MMP9	1.0 ± 0.5	1.5 ± 0.8	2.6 ± 1.1
CXCL10	1.3 ± 0.8	61.4 ± 26.0*	5.0 ± 1.8*	NOTCH4	1.6 ± 0.3	1.3 ± 0.5	1.7 ± 0.3
CXCL3	9.1 ± 4.4*	16.6 ± 4.7*	101.4 ± 17.3*	NRP1	0.7 ± 0.1	1.0 ± 0.1	0.7 ± 0.1
CXCL5	2.2 ± 0.4	2.6 ± 0.5	25.6 ± 7.8*	NRP2	0.5 ± 0.1*	1.1 ± 0.1	0.6 ± 0.1
CXCL6	3.9 ± 0.3	37.3 ± 17.0*	138.9 ± 55.4*	PDGFA	1.0 ± 0.1	0.9 ± 0.1	1.4 ± 0.3
CXCL8	32.0 ± 23.0*	16.8 ± 0.5*	301.6 ± 20.0*	PECAM1	3.5 ± 0.4	0.9 ± 0.1	3.1 ± 0.7
CXCL9	3.3 ± 0.4	0.7 ± 0.2	2.1 ± 0.7	PF4	1.8 ± 0.7	0.8 ± 0.2	2.4 ± 1.2
ECGF1	0.4 ± 0.1*	2.2 ± 0.1*	0.5 ± 0.1*	PGF	3.7 ± 0.6*	0.7 ± 0.3	4.1 ± 0.6*
EDG1	0.5 ± 0.1*	0.9 ± 0.2	0.5 ± 0.1*	PLAU	0.2 ± 0.1*	1.0 ± 0.1	0.2 ± 0.1*
EFNA1	2.2 ± 0.4	0.9 ± 0.3	2.7 ± 1.1*	PLG	2.4 ± 0.2	1.1 ± 0.4	1.8 ± 0.6
EFNA3	8.2 ± 2.2*	1.0 ± 0.2	8.0 ± 2.0*	PLXDC1	2.2 ± 0.4	1.1 ± 0.2	1.7 ± 0.5
EFNB2	1.6 ± 0.4	1.1 ± 0.2	1.5 ± 0.5	PROK2	2.4 ± 0.4	1.4 ± 0.7	2.2 ± 0.4
EGF	1.9 ± 0.6	0.7 ± 0.1	1.5 ± 0.2	PTGS1	1.1 ± 0.3	1.0 ± 0.2	1.2 ± 0.5
ENG	0.7 ± 0.1	1.1 ± 0.2	0.8 ± 0.1	SERPINF1	0.7 ± 0.2	1.0 ± 0.2	0.7 ± 0.2
EPHB4	0.9 ± 0.1	0.9 ± 0.1	0.8 ± 0.1	SPHK1	1.5 ± 0.1*	1.1 ± 0.2	1.7 ± 0.2*
EREG	2.4 ± 0.8	1.0 ± 0.1	1.3 ± 0.3	STAB1	2.2 ± 0.4	1.0 ± 0.4	2.5 ± 0.7
FGF1	0.3 ± 0.1	0.9 ± 0.1	0.3 ± 0.1	TEK	0.9 ± 0.2	1.3 ± 0.6	0.7 ± 0.2
FGF2	1.8 ± 0.3	1.2 ± 0.2	2.2 ± 0.5	TGFA	2.7 ± 0.4	1.3 ± 0.6	1.9 ± 0.4
FGFR3	2.0 ± 0.4	0.8 ± 0.1	1.4 ± 0.4	TGFB1	2.1 ± 0.2*	0.9 ± 0.1	2.4 ± 0.2*
FIGF	1.2 ± 0.3	1.1 ± 0.2	0.9 ± 0.3	TGFB2	0.7 ± 0.1	0.9 ± 0.1	0.6 ± 0.1
FLT1	1.2 ± 0.3	1.3 ± 0.4	1.2 ± 0.2	TGFBR1	0.5 ± 0.1*	1.0 ± 0.1	0.7 ± 0.1
HAND2	2.6 ± 0.2	1.5 ± 0.8	2.6 ± 0.9	THBS1	0.8 ± 0.1	1.1 ± 0.1	0.7 ± 0.1
HGF	0.6 ± 0.1	1.1 ± 0.3	0.7 ± 0.2	THBS2	2.2 ± 0.4*	1.1 ± 0.2	2.3 ± 0.3*
HIF1A	0.5 ± 0.1*	1.0 ± 0.2	0.5 ± 0.1*	TIMP1	1.4 ± 0.3	1.0 ± 0.2	1.5 ± 0.2
HPSE	1.9 ± 0.3	1.2 ± 0.2	1.8 ± 0.1	TIMP2	0.7 ± 0.1	0.9 ± 0.1	0.7 ± 0.1
ID1	0.9 ± 0.1	1.0 ± 0.3	0.7 ± 0.1	TIMP3	2.3 ± 0.8	1.4 ± 0.5	1.5 ± 0.4
ID3	0.7 ± 0.4	0.8 ± 0.3	0.6 ± 0.2	TNF	2.7 ± 0.1	1.0 ± 0.3	2.2 ± 0.5
IFNA1	4.6 ± 2.0	1.1 ± 0.4	7.4 ± 5.2	TNFAIP2	1.2 ± 0.3	3.9 ± 0.5*	2.7 ± 0.3
IFNB1	2.0 ± 0.7	0.7 ± 0.2	1.2 ± 0.4	VEGF	41.3 ± 16.5*	1.0 ± 0.2	47.1 ± 10.3*
IFNG	1.2 ± 0.3	1.2 ± 0.3	1.2 ± 0.1	VEGFC	3.1 ± 0.7	1.0 ± 0.1	3.1 ± 0.3

Note. EFNA3, ephrin-A3; PGF, placental growth factor.

^aData represent mean ± SEM of Ni and MALP-2 induced fold-change in gene expression relative to control-treated cells (n = 3), *p < 0.05.