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. 2008 Oct 16;107(1):144–155. doi: 10.1093/toxsci/kfn211

FIG. 1.

FIG. 1.

Blots showing FP-biotin–reactive proteins in human (A) and mouse (B) plasma. FP-biotinylated plasma proteins were separated on nondenaturing polyacrylamide gels and transferred to PVDF membranes. Blots were hybridized with the fluorescent probe Streptavidin Alexa Fluor 680. A1, 20 μg human transferrin; A2, blank; A3, 20 μg FP-biotinylated human transferrin; A4, blank; A5, 5 μl of human plasma; A6, blank; A7, 3.3 μl of FP-biotinylated human plasma; A8, 6.6 μl of FP-biotinylated human plasma; A9, 9.9 μl of FP-biotinylated human plasma; A10, blank; A11, 1 pmol FP-biotinylated human butyrylcholinesterase (BChE). B1, 20 μg mouse transferrin; B2, blank; B3, 20 μg FP-biotinylated mouse transferrin; B4, blank; B5, 5 μl of mouse plasma; B6, blank; B7, 5 μl of FP-biotinylated mouse plasma; B8, 10 μl of FP-biotinylated mouse plasma; B9, 15 μl of FP-biotinylated mouse plasma. The heavy band in B contains mouse ES1 carboxylesterase and mouse albumin. ES1 carboxylesterase in mouse plasma does not separate well from albumin on a nondenaturing gel. Human plasma does not contain carboxylesterase.