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. 2008 Oct 16;107(1):144–155. doi: 10.1093/toxsci/kfn211

FIG. 2.

FIG. 2.

Covalent binding of FP-biotin to human alpha-1-antitrypsin, human complement C3, human alpha-2-macroglobulin, and bovine holo-transferrin. (A) Coomassie stained SDS gel. (B) Blot hybridized with Streptavidin Alexa Fluor 680 to visualize FP-biotinylated proteins transferred to PVDF membrane from the gel. Lane 1, FP-biotinylated alpha-1-antrypsin; lane 2, unlabeled alpha-1-antitrypsin; lane 3, FP-biotinylated complement C3; lane 4, unlabeled complement C3; lane 5, FP-biotinylated alpha-2-macroglobulin; lane 6, unlabeled alpha-2-macroglobulin; lane 7, FP-biotinylated transferrin; lane 8, unlabeled transferrin.