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Labeling of peptide ArgTyrThrArg by dichlorvos, chlorpyrifos oxon, and DFP. The reaction of 170μM peptide with 0.1, 0.2, or 1mM OP was in ammonium bicarbonate buffer pH 8.3, at 37°C for 16 h. MALDI-TOF cluster areas were used to calculate % labeling. MS/MS spectra identified the labeled residue as tyrosine.
