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. 2008 Oct 16;107(1):144–155. doi: 10.1093/toxsci/kfn211

FIG. 8.

FIG. 8.

FP-biotinylation of Tyr 238 and Tyr 574 in human transferrin does not interfere with ferric ion binding. One milliliter of 12.1μM apo-transferrin control (♦) titrated with FENTA had an endpoint of 27.3μM FENTA, which calculates to 2.26 mol of ferric ion bound per mole of transferrin. One milliliter of 14.67 μM FP-biotinylated apo-transferrin (▪) had an endpoint of 31.3μM FENTA, which calculates to 2.11 mol of ferric ion bound per mole of transferrin. Assays for each type of transferrin were performed in duplicate.