TABLE 1.
OP | Human-labeled peptide | Mouse-labeled peptide | OP-Tyr |
FP-biotin | KPVDEY*K | KPVDQY*EDCYLAR | Y238 |
FP-biotin | LYLGHNY*VTAIR | Y319 | |
FP-biotin | GY*YAVAVVK | Y429 | |
FP-biotin | FDEFFSQGCAPGY*EK | Y491 | |
FP-biotin | EEYNGY*TGAFR | Y518 | |
FP-biotin | KPVEEY*ANCHLAR | Y574 | |
DFP | KPVDEY*K | KPVDQY*EDCYLAR | Y238 |
DFP | GY*YAVAVVK | Y429 | |
DFP | KPVEEY*ANCHLAR | Y574 | |
CPO | KPVDEY*K | KPVDQY*EDCYLAR | Y238 |
CPO | LYLGHNY*VTAIR | Y319 | |
CPO | GY*YAVAVVK | Y429 | |
CPO | FDEFFSQGCAPGY*EK | Y491 | |
CPO | EEYNGY*TGAFR | Y518 | |
CPO | KPVEEY*ANCHLAR | Y574 | |
Dichlorvos | KPVDEY*K | Y238 | |
Dichlorvos | KPVEEY*ANCHLAR | Y574 | |
Sarin | KPVDEY*K | KPVDQY*EDCYLAR | Y238 |
Sarin | GY*YAVAVVK | Y429 | |
Sarin | GYY*AVAVVK | Y430 | |
Sarin | KPVEEY*ANCHLAR | Y574 | |
Soman | KPVDEY*K | KPVDQY*EDCYLAR | Y238 |
Soman | GYY*AVAVVK | Y430 | |
Soman | KPVEEY*ANCHLAR | Y574 |
Note. Single letter codes for amino acids are A, ala; C, cys; D, asp; E, glu; F, phe; G, gly; H, his; I, ile, K, lys; L, leu; M, met; N, asn; P, pro; Q, gln; R, arg; S, ser; T, thr; V, val; W, trp; Y, tyr. The labeled tyrosine is designated by an asterisk Y*. The table shows the amino acid sequences of the tryptic peptides containing OP-labeled tyrosine. The location of the labeled tyrosine in the transferrin protein is given in the last column. Tyrosine 238 of transferrin was labeled in both species. Tyr 319, 429, 430, 491, and 518 was labeled only in mouse transferrin. Tyr 574 was labeled only in human transferrin. Transferrin 1 mg/ml in pH 8.5 buffer was treated in vitro with 0.5mM OP at 37°C for 16 h.