TABLE 2.

Effect of DE-71 on Hepatic Cytochrome P450 Gene Expression and Protein Activity

Target	Treatment (mg/kg/day)	PND 4	PND 21	PND 60
Cyp1a1a	0	1.6 ± 0.2	8.2 ± 2.5	0.02 ± 0.0
	1.7	26.0 ± 9.9*	38.1 ± 11.6**	0.02 ± 0.0
	10.2	71.3 ± 19.7*	84.1 ± 24.8*	0.04 ± 0.0
	30.6	248.3 ± 29*	165.8 ± 55.6*	0.10 ± 0.1**
ERODb	0	4.2 ± 1.1	108.9 ± 12	87.2 ± 9.0
	1.7	98.2 ± 13.4**	443.2 ± 50.4**	84.3 ± 7.2
	10.2	208.1 ± 62.3**	1872.0 ± 203.2**	83.2 ± 5.1
	30.6	904.1 ± 113.2**	3472.2 ± 439.1**	85.7 ± 6.8
Cyp2b1a	0	1.7 ± 0.1	5.5 ± 0.8	0.3 ± 0.0
	1.7	6.6 ± 2.7**	37.7 ± 17.3**	0.4 ± 0.2
	10.2	21.6 ± 7.4**	89.7 ± 33.2*	0.5 ± 0.1
	30.6	33.7 ± 6.8*	147.7 ± 20.1*	0.5 ± 0.1
Cyp2b2a	0	1.7 ± 0.3	6.5 ± 1.5	0.7 ± 0.1
	1.7	3.4 ± 1.74	27.5 ± 5.6**	1.0 ± 0.3
	10.2	9.6 ± 3.5**	42.8 ± 9.8**	1.0 ± 0.2
	30.6	12.1 ± 2.7*	69.6 ± 13.2*	0.9 ± 0.1
PRODb	0	0.4 ± 0.2	21 ± 7.2	11.2 ± 4.2
	1.7	3.5 ± 1.2**	108 ± 12.4**	12.3 ± 3.1
	10.2	18.9 ± 5.5*	219 ± 48.2*	13.2 ± 2.9
	30.6	22.2 ± 6.7*	202 ± 42.8*	11.7 ± 4.6
Cyp3a1a	0	1.5 ± 1.1	1.1 ± 0.8	0.5 ± 0.1
	1.7	1.0 ± 0.4	1.2 ± 0.8	0.6 ± 0.1
	10.2	2.4 ± 1.0	7.6 ± 2.6**	0.7 ± 0.1
	30.6	3.7 ± 2.2**	16.3 ± 5.8*	0.6 ± 0.1
BRODb	0	15.1 ± 11.2	52.1 ± 22.8	25.3 ± 10.1
	1.7	35.0 ± 10.7	301.0 ± 100.7**	25.9 ± 11.2
	10.2	148.3 ± 42.0**	1818.4 ± 420.1*	26.2 ± 12.8
	30.6	201.1 ± 82.5*	2120.5 ± 582.5*	26.3 ± 11.1

Note. Phase I hepatic microsomal EROD, PROD, and BROD activity along with real-time PCR of Cyp1a1, Cyp2b1, Cyp2b2, and Cyp3a1 gene expression were measured. Microsomes and RNA were isolated from male rat livers at PND 4, 21, and 60 after perinatal exposure to the polybrominated diphenyl ether mixture, DE-71 (0, 1.7, 10.2, and 30.6 mg/kg/day) from GD 6 to PND 21. Significantly different from control: (p < 0.01)* or (p < 0.05)**.

^aData are expressed as relative quantitation mean ± SD.

^bData are expressed as pmol/mg protein/min ± SD, n = 5.