Fig. 3.

Retinoid profiles of Rpe65^-/- and WT mice. HPLC chromatograms of retinoid extractions from Rpe65^-/- and WT mice (cyclic-light-reared from birth for 2 months, then dark-reared for 4 weeks, three eyes per sample). (A) Rpe65^-/- mouse retina. (B) Rpe65^-/- mouse RPE fraction. (C) WT mouse retina. (D) WT mouse RPE fraction. (B and D Insets) Full-scale chromatograms. Peaks were identified by comparison to known standards. Peak 1, all-trans-retinyl esters; peak 2, syn-9-cis-retinal oxime; peak 2′, anti-9-cis-retinal oxime; peak 3, syn-11-cis-retinal oxime; peak 3′, anti-11-cis-retinal oxime; peak 4, syn-all-trans-retinal oxime; peak 4′, anti-all-trans-retinal oxime; peak 5, syn-13-cis-retinal oxime; peak 5′, anti-13-cis-retinal oxime. Detection is at 360 nm.