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Effect of antioxidant on cell death induced by B deprivation. After pre-culture for 1 h in a standard growth medium supplemented with 0.1 mM of either BHA (a) or α-tocopherol (b), cells were washed with and transferred to a B-free medium supplemented with the same 0.1 mM antioxidant. Cells treated in the same way but without antioxidant were prepared as a control. Each bar shows the mean of results from four randomly selected fields ± SD.
