Fig. 2.

⁵²Fe translocation in Fe-deficient (−Fe) and Fe-sufficient (+Fe) barley in the dark and in the light (Experiment 1). (A–D) Images of ⁵²Fe translocation in Fe-deficient (A, B) and Fe-sufficient (C, D) barley in the dark (A, C) and in the light (B, D) using BAS-1500. Leaf numbering: 4, youngest leaf (YL); 3, third leaf; 2, second leaf; 1, first leaf. (E and F) Time course of ⁵²Fe translocation in the DC (E) and at the points indicated in A and B (F) of Fe-deficient barley measured by PMPS and PETIS, respectively. The maximum measured radioactivity in PMPS and PETIS was defined as 100. Activities are shown as percentages of the maximum value. (G) ⁵²Fe content in the leaves of Fe-deficient barley after 6 h.