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. 2008 Dec 10;50(1):13–25. doi: 10.1093/pcp/pcn186

Fig. 3.

Fig. 3.

Growth of qko-35S-AtAMT1;4 transgenic lines on ammonium and its toxic analog methylammonium (MeA). (A) Growth of qko plants and transgenic qko-35S:AtAMT1;4 plants (lines H2f, H3a and H8g) on agar containing 250 μ M NH4+ (125 μ M as ammonium succinate) or 250 μ M NO3− (250 μ M as potassium nitrate) for 10 d after pre-culture on half-strength MS medium (containing 5 mM nitrate as sole nitrogen source) for 7 d. (B) Shoot dry weights of the same plants as in (A). Bars indicate means ± SD (n = 6), and significant differences at P < 0.001 within each group are indicated by different letters. (C) Growth of qko plants and transgenic qko-35S:AtAMT1;4 plants (lines H2f, H3a and H8g) on agar containing no or 10 mM MeA in the presence 1 mM nitrate and 1% sucrose for 10 d after pre-culture on half-strength MS medium (containing 5 mM nitrate as sole nitrogen source) for 8 d. (D) Shoot dry weights of the same plants as in (C). Bars indicate means ± SD (n = 4), and significant differences at P < 0.001 within each group are indicated by different letters.