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. 2008 Dec 10;50(1):13–25. doi: 10.1093/pcp/pcn186

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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.

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Fig. 6. — AtAMT1;4 localizes to the plasma membrane of pollen grains and pollen tubes. Fluorescence microscopy of (A) a mature pollen grain and (B) a pollen tube of transgenic plants expressing the AtAMT1;4-promoter:ORF:GFP construct or (C and D) expressing the AtAMT1;4-promoter:GFP construct. GFP fluorescence micrographs in (A–D) were present on a black background, alongside equivalent light micrographs on a gray background. Flowers and pollen grains were harvested from 6-week-old plants grown in soil, and pollen tubes were germinated in vitro.