Fig. 2.

Salt-induced LePLDα1 activity in tomato cell suspension cultures. ³²P_i-labeled control or LePLDα1-silenced (line 1) cell suspensions were left untreated, snap-frozen and thawed or treated with an equal volume of cell-free medium supplemented with increasing NaCl concentrations for 15 min, either without buffer or buffered with 50 mM Tris–HCl pH 7.5 and 10 mM EGTA (TE). Lipids were extracted, separated by TLC and analyzed by phosphoimaging. PA (a and b) and PBut (c and d) were quantified as a percentage of total radiolabeled lipids and are presented in a histogram (salt treatment n = 2, min and max values indicated; freeze/thaw n = 1).