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. 2008 Nov 18;50(1):78–89. doi: 10.1093/pcp/pcn173

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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.

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Fig. 4. — Salt tolerance and lysis-induced PLD activity in LePLDα1-silenced tomato plants. (a) Tomato seeds from wild-type and LePLDα1-silenced plant lines were sown on agar plates and grown vertically in a growth chamber. After 1 week, seedlings were transferred to fresh plates supplemented with 0, 125 or 250 mM NaCl, rotated 180° and placed back in the growth chamber. Plates were scanned after 24 h. A representative silenced line is shown (line #3). (b) Leaf discs were excised from fully expanded leaves from wild-type and LePLDα1-silenced (line #13) tomato plants as well as wild-type and pldα1 knock-out Arabidopsis plants, and labeled overnight with ³²P_i. Leaf discs were either left untreated or snap-frozen and thawed for 15 min. Lipids were extracted, separated by TLC and analyzed by phosphoimaging. PA was quantified as a percentage of total phospholipids and is presented in histograms ± SD (n = 3).