Fig. 5.
Salt-induced PLD activity in Arabidopsis T-DNA insertion lines. Leaf discs were excised from fully expanded leaves from control and pld knock-out plant lines and labeled overnight with 32Pi. Leaf discs were treated with increasing NaCl concentrations for 15 min. Lipids were extracted, separated by alkaline TLC (a) and analyzed by phosphoimaging (b). PA was quantified as a percentage of total radiolabeled lipids and is presented in a histogram ± SD (n = 3).