Fig. 7.

Dehydration-induced PLD activity in Arabidopsis T-DNA insertion lines. Leaf discs were excised from fully expanded leaves from control and pld knock-out plant lines and labeled overnight with ³²P_i. Leaf discs were treated by removing them from the labeling solution and placing them on filtration paper for 2 h. Lipids were extracted, separated by ethyl acetate TLC (a) and analyzed by phosphoimaging (b). PA was quantified as a percentage of total radiolabeled lipids and is presented in a histogram ± SD (n = 3).