Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 17;50(1):58–66. doi: 10.1093/pcp/pcn168

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

PMC Copyright notice

Fig. 2. — Generation and growth characterization of Pro35S-NIP5;1:NIP5;1 transgenic plants in the nip5;1-1 background. (A) The schematic representation of the DNA construct which has the CaMV 35S promoter at 1,357 bp upstream of the NIP5;1 transcription initiation site. (B) Relative mRNA level of NIP5;1 in roots of the transgenic plants. Plants were grown on plates containing 100 μM B for 12 d. Then the plants were transferred to plates containing 0.1 μM B and incubated for 1 d. Total RNAs were extracted from roots of 7–10 plants. The averages and SDs are presented for independent reverse transcription reactions followed by real-time PCR (n = 3). (C–F) Growth of the transgenic plants. Plants were grown on solid medium containing 100 or 0.1 μM boric acid for 12 d. Shoot fresh weights and primary root lengths were measured. Averages and SDs are shown (n = 9–10). Asterisks indicate significant differences from the wild-type plants (Student's t-test P < 0.05). (C) Primary root length under 100 μM B conditions. (D) Shoot fresh weight under 100 μM B conditions. (E) Primary root length under 0.1 μM B conditions. (F) Shoot fresh weight under 0.1 μM B conditions.