Fig. 1.

CR ameliorates the clinical course of EAE in SJL and C57BL/6 mice. (A) Body weights of control mice (•), mice fed with HF diet (▪), and CR mice (○) throughout the experiment before and after immunization (values are mean±sd). The weight changes shown are representative of four experiments. The dotted vertical line indicates the day of immunization. (B) Experiment 1. Survival was increased significantly in CR mice (80%) compared with control (23%) and HF (30%) groups (P=0.02 CR vs. control by χ²). (C) Experiment 3. CR regimen reduces the clinical severity of relapsing-remitting EAE in SJL mice compared with the ad libitum diet in control mice. (D) Experiment 4. CR ameliorates the clinical course of chronic-progressive EAE in C57BL/6 mice. (C and D) Values are mean ± sem; clinical scores were calculated as the average of all animals in each group.