Fig. 4.
Chronic CR does not inhibit T cell priming in the EAE-induction phase. In vitro proliferation rate (A) and IFN-γ production (B) in response to PLP139–151 by cells isolated 10 days p.i. from draining lymph nodes (axillary and inguinal) did not show any significant differences amongst SJL controls, mice assigned to a HF or CR (n=2/group; all mice were Clinical Grade 0 except one in the control group that was Grade 2). Proliferation and cytokine production in response to PLP139–151 by splenocytes isolated from SJL mice 30 days p.i. was also tested (n=5/group; control mice were at clinical scores ranging from 2 to 4, two CR mice were at score 0, two were at 2, and one at score 1; C and D). Neither splenocyte proliferation in vitro nor cytokine production showed significant differences between the control and CR groups. Values are mean ± sem. These data are representative of four experiments.