Figure 2.

Comparison of the intracellular localization of NPR-B wild-type and patient mutant variants (extracellular ligand-binding domain) with the ER. HeLa cells were transiently transfected with plasmids encoding the indicated HA-tagged NPR-B proteins, fixed and stained with antibodies as described in Materials and Methods. (A), (D), (G), (J), (M), (P), (S) and (V) show the distribution of overexpressed HA-tagged NPR-B protein, (B), (E), (H), (K), (N), (Q), (T) and (W) show the distribution of endogenous ER-resident calnexin, whereas (C), (F), (I), (L), (O), (R), (U) and (X) show the extent of co-localization of NPR-B proteins and calnexin. Bars: 20 µm.