Figure 4.

Comparison of the intracellular localization of NPR-B wild-type and patient mutant variants (intracellular KHD and GCD) with the ER. HeLa cells were transiently transfected with plasmids encoding the indicated HA-tagged NPR-B proteins, fixed and stained with antibodies as described in Materials and Methods. (A), (D), (G), (J), (M) and (P) show the distribution of overexpressed HA-tagged NPR-B protein, (B), (E), (H), (K), (N) and (Q) show the distribution of endogenous ER-resident calnexin, whereas (C), (F), (I), (L), (O) and (R) show the extent of co-localization of NPR-B proteins and calnexin. Bars: 20 µm.