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. 2008 Oct 17;18(2):248–260. doi: 10.1093/hmg/ddn344

Figure 2.

Figure 2.

Necdin activates GnRH gene expression. (A) Two luciferase reporters were studied: −5 kb GnRH and GnRH enhancer reporter, which is a condensed combination of the three well-characterized rat GnRH regulatory elements: upstream enhancer (UE), enhancer (E) and promoter (P) (36). (B) Each reporter was co-transfected into GN11 cells with a necdin expression vector (necdin) or empty vector (control). (C) GT1-7 cells were co-transfected with pGL3-luciferase or GnRH enhancer reporter and either necdin expression vector or empty vector control. (D) GN11 cells were co-transfected with GnRH enhancer reporter and either necdin, Magel2, Dlxin-1, or empty vector control. Experiments were performed in quadruplicate and repeated at least three times. Results shown are mean ± SEM. The means were compared by one-way ANOVA and Tukey–Kramer HSD. *indicates significant difference (P < 0.05) relative to empty vector control.