Figure 3.

Necdin specifically disrupts Msx repression of GnRH gene expression. (A) The GnRH enhancer reporter was co-transfected into GN11 cells with either empty vector control or Msx1 expression vector ± necdin expression vector. *Significant difference (P < 0.05) from empty vector control, while ^#Significant difference (P < 0.05) relative to Msx1 alone, as indicated by brackets. (B) The GnRH enhancer reporter was co-transfected with either empty vector control, or with Dlx1, Dlx2 and Dlx5 expression vectors (Dlx) ± necdin expression vector. Experiments were performed in quadruplicate and repeated at least three times. Results shown are mean ± SEM. One-way ANOVA and Tukey–Kramer HSD compared the means.