Figure 6.

Necdin interacts with Msx in GT1-7 cells. (A) Proteins from GT1-7 cells were immunoprecipitated (IP) with either anti-necdin antibody or rabbit IgG control. Msx1 and necdin were detected by immunoblotting the immunoprecipitated proteins. Ten percent of the input was used for Msx1 and 2% for necdin. (B) GT1-7 cells were transiently transfected with Msx1-FLAG, Dlxin1-FLAG expression vector or empty vector control. Cell extract was immunoprecipitated with anti-FLAG affinity gel. Immunoprecipitates and 4% of the input were immunoblotted for necdin.