Skip to main content
. 2008 Oct 16;18(2):241–247. doi: 10.1093/hmg/ddn343

Figure 2.

Figure 2.

Effect of MEK inhibitor PD98059 on cardiac expression of natriuretic peptides and myosins in LmnaH222P/H222P mice. (A) Immunoblot showing the expression of natriuretic peptide precursor A (Nppa) in hearts from LmnaH222P/H222P mice treated with PD98059 or placebo (DMSO). Results using hearts from Lmna+/+ mice and untreated LmnaH222P/H222P mice are shown for comparison. Labeling with antibody against Gapdh is shown as a loading control. (B) Quantitative real-time RT–PCR showing the expression of RNAs from NppA and NppB genes, respectively, encoding natriuretic peptide precursors A and B, and Myl4 and Myl7 genes, encoding myosin light chains, in hearts from LmnaH222P/H222P mice treated with PD98059 or placebo (DMSO). Results from hearts from Lmna+/+ mice and untreated LmnaH222P/H222P mice are shown for comparison. Bars indicate the fold overexpression of the indicated mRNA in hearts as calculated by the CT method. Values are means ± standard deviations for n = 4 samples from different animals per group. Reactions were performed in triplicate for each different RNA sample. Results were compared using a two-tailed t-test (*P < 0.05).