Figure 1.

Generation of dUBE3A mutant alleles. (A) Genomic organization of the dUBE3A locus. dUBE3A contains eight exons and is located on the chromosome 3L and flanked by genes CG6199 and CG7600. Arrows indicate the direction of transcription. (B) Schematic of dUBE3A mutant alleles generated with the P-element local hop-out approach. An EP element is inserted in the first exon of dUBE3A. Excision of this P-element resulted in the generation of deletion mutant alleles of dUBE3A. The size of each deletion was determined by the sequencing of PCR fragments covering the deletion sites. In line Δ15-8, a 1156 bp fragment from the P-element remained in the first exon of dUBE3A, creating an insertional mutant allele.