Figure 2.

Absence of dUBE3A expression in the mutant alleles. (A) Quantitative PCR (qPCR) analysis of relative mRNA levels of dUBE3A and CG7600 in adult flies. Values are mean ± SEM. (B) Expression levels of dUBE3A protein in fly head extracts. Fly heads were used for western blot analysis because smaller dUBE3A fragments, presumably due to non-specific degradation, were observed when protein extracts from whole flies were used (not shown). This polyclonal antibody recognized several non-specific bands on western blot. However, the dUBE3A band of the predicted molecular weight was absent.