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. 2008 Jun 11;19(3):511–523. doi: 10.1093/cercor/bhn101

Figure 2.

Figure 2.

Vesicular density in stimulated and control MF terminals in synapsin WT and DKO mice. (A) Electron micrograph of a MF terminal (t) from a WT hippocampal slice preparation. A synapse is indicated by its postsynaptic density (arrowheads), spine (s), vesicles (v), and mitochondria (m). Scale bar: 0.1 μm. (B) As in (A), but the electron micrograph is from a synapsin DKO MF terminal. (C, D) As in (A) and (B), respectively, but the electron micrographs are from slices exposed to 30 mM [KCl]o. Artifactual small, electron-dense precipitates are present in electron micrographs (A–D). (E) Vesicle densities (mean + standard error of the mean, vesicles/μm2, n = 3 mice) plotted against distance from the presynaptic membrane specialization in WT and synapsin DKO mice, either in control situation or exposed to high [KCl]o. Vesicles were grouped into bins of 50 nm and were recorded up to a maximum distance of 400 nm. (F) As in (E), but results are grouped into distances of 0- to 100-nm distance and 0–400 nm, corresponding to RRP and RP, respectively. Vesicle densities were compared between genotypes at the given distances (#P < 0.01). This could not be confirmed with a Mann–Whitney test because of the low n. For each condition, 3 WT and 3 synapsin DKO mice (n = 3) were analyzed and compared (20 electromicrographs containing 18–45 synapses were analyzed in each mouse).