Fig. 5.
IL-1β and TSLP protein levels are reduced 4 h after FITC challenge by Cmpd A. Mice were treated with either drug Veh or Cmpd A (1 mg kg−1) 1 h before FITC challenge to the right ear. The left ears were challenged with FITC Veh (acetone:dibutyl phthalate). After 4 h, the ears were harvested and the protein lysates from the right ears were assayed for (A) IL-1β (50 μg total protein) and (B) TSLP (100 μg total protein). As TSLP levels were high in the Veh/veh group, the left ears (Veh challenged) from mice in all the groups were assayed for TSLP protein levels (C). Additionally, six ears from untreated BALB/c mice were also examined for TSLP protein levels. A minimum of five animals were tested per condition except where noted above, and the results show the mean ± SEM. ***P < 0.001, *P < 0.05.