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. 2008 Dec 23;21(2):155–165. doi: 10.1093/intimm/dxn133

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© The Author 2008. Published by Oxford University Press on behalf of The Japanese Society for Immunology. All rights reserved.

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Fig. 5. — Ex vivo correlation of [¹⁸F]FHBG retention with numbers of Vβ13+ sr39tk+ cells present in the spleen and LNs. (A) General schematic. Mice were injected i.v. with [¹⁸F]FHBG on day 3 post-AT. Immediately after whole-body imaging, spleen and LNs were isolated, (B) imaged ex vivo by micro-PET, counted with the gamma counter and cells were analyzed by FACS. Mice receiving partial treatment (XRT + AT) and combined immunotherapy group (XRT + AT + IL-2 + DC) were compared. (C) Increased numbers of live Vβ13+ sr39tk+ T cells in the organs of mice from the combined immunotherapy group (XRT + AT + IL-2 + DC) compared with the XRT + AT group. Correlation of in vivo [¹⁸F]FHBG retention with the numbers of Vβ13+ sr39tk+ cells present in (D) spleen and (E) LNs.