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RHBDF1 siRNA inhibits the release of TGF-α by the 1483 cells determined by ELISA. A) TGF-α concentrations in conditioned medium of GRP-stimulated cells (solid bars) relative to control cells without GRP treatment (open bars). B) Levels of TGF-α protein associated with GRP-stimulated cells treated with either the control siRNA (Con siRNA) or the RHBDF1 siRNA. Data are from 3 independent experiments. *P < 0.01, Student’s t test.
