FIGURE 3. Müller glia upregulated cyclin D3 and migrated to the IPL and ONL after α-AA stimulation.
(A–C) Photomicrographs of retinal sections taken at 8 hours after α-AA treatment and double-labeled by anti-BrdU (green) and glutamine synthetase (GS; red). (D–H) Retinal sections collected 6 hours after saline (D) or α-AA (E–I) injection were immunolabeled with a primary antibody against cyclin D3 (D, E, G) and counterstained with a green nuclear marker (H, I). Arrows: cell nuclei that migrated into the ONL (H–I). Note that the missing cyclin D3+ nuclei in the INL appeared in the IPL and ONL. Scale bars, 40 µm.