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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1142–1150. doi: 10.1167/iovs.07-0434

FIGURE 5. Glutamate induced Müller glial proliferation in purified cell cultures.

FIGURE 5

(A, B) Photomicrographs of cultured Müller glial cells that were double-stained with DAPI and antibodies against CRALBP (A) or GFAP (B). (C–H) Photomicrographs of Müller glial cultures treated with (C–E) control solution or (F–H) L-glutamate (0.5 mM) and double-stained with DAPI and antibodies against BrdU. (I) Incorporation of BrdU 48 hours after treatment with saline, L-glutamate, or α-AA. Data are the mean ± SD. *P < 0.001 versus control (two-tailed t-test). Scale bar, 10 µm.

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