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. 2008 Nov;59(15):4271–4287. doi: 10.1093/jxb/ern276

Fig. 5.

Fig. 5.

Characterization of SlTPR1-overexpressing tomato plants. (A) Overexpression construct of SlTPR1. (B) Northern analysis to determine the SlTPR1 transgene expression in tomato primary transformants. Total RNA from both transgenic and wild-type plants was blotted and probed with the SlTPR1 cDNA. (C) Northern analysis to determine the mRNA levels of both endogenous and transgene SlTPR1 in the transgenic lines (the transgene SlTPR1 has a GFP-tag and is larger than the endogenous mRNA). 10 μg total RNA from the vegetative buds was blotted and probed with the SlTPR1 cDNA. The ethidium bromide-stained rRNA below indicates sample loading. All the samples were run in the same gel, but the order of the lanes was rearranged to correspond to the expression level of the transgene and to aid comparison with Fig. 5D. (D) Phenotypes of SlTPR1 transgenic plants. Photographs were taken of plants 80 d after transferring from tissue culture to compost. (E) A close-up of plants in (d) to show inhibited apical dominance and altered leaves of line 3286A compared with the wild type. (F, G) Leaf morphology of the transgenic plants compared with the wild type.