Table 1.
Generation of HIV-neo pseudotypes
| Cells transfected | Virus envelope | p24, ng/ml | Virus
titer on cells,* cfu/ml
|
||
|---|---|---|---|---|---|
| 3T3 | HOS | TE671 | |||
| 293T | None | 242 | <101 | <101 | <101 |
| Mo-MLV-A | 278 | 6.1 × 106 | 6.0 × 106 | 7.8 × 106 | |
| VSV-G (SV-GL) | 44 | 1.2 × 105 | 1.1 × 106 | 8.0 × 105 | |
| VSV-G (LTR-G) | 95 | 2.3 × 107 | 3.1 × 107 | 3.1 × 107 | |
| BOSC-23 | Mo-MLV-E | 278 | 5.3 × 106 | NA | NA |
| BING | Mo-MLV-A | 266 | 1.7 × 105 | NA | NA |
| COS-7 | Mo-MLV-A | 27.5 | 5.8 × 106 | 4.2 × 106 | 3.7 × 106 |
| VSV-G (SV-GL) | 7.9 | 2.0 × 106 | 3.0 × 105 | 6.0 × 105 | |
| VSV-G (LTR-G) | 6.7 | 2.2 × 106 | 2.0 × 106 | 4.0 × 106 | |
NA, not available; cfu, colony-forming units.
*
Infected cells were trypsinized 3 days after transduction and cultured for 10–14 days in DMEM/FBS containing G418 (0.35 mg/ml active drug). Colonies were stained using 0.2% crystal violet in 20% ethanol. Data from one representative experiment of several are shown.