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. 2008 Sep 11;24(1):85–96. doi: 10.1093/ndt/gfn433

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© The Author [2008].

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Fig. 9 — Graph representing appropriate protein band HIF-1α (A) from western blot analysis and pro and active MMP-9 from zymography (B) with blood outgrowth endothelial cell (BOEC) delivery compared to control (C). According to semiquantitative analysis, by Day 14, the normalized density of HIF-1α was significantly less in the BOEC samples when compared to control (P < 0.05) while the normalized density of both pro and active MMP-9 was significantly higher than the control vessels (P < 0.05). (D), (E) Confocal double immunostaining of MMP-9-positive cells (red) from BOEC-transplanted vessels and (F) the contralateral non-transplanted (control) vessel. Ad is the adventitia, M is the media, N is the neointima and L is the lumen. ^*An adventitial microvessel surrounded by cells expressing MMP-9. More intense immunostaining was observed in the neointima of the transplanted vessels when compared to non-transplanted vessels.